mpc protein precipitation solution Search Results


biotinylated fusion proteins for the wild-type or mutated xa-2-b3ar loop3 or xa-2-b3ar tail  (Promega)
90
Promega biotinylated fusion proteins for the wild-type or mutated xa-2-b3ar loop3 or xa-2-b3ar tail
Biotinylated Fusion Proteins For The Wild Type Or Mutated Xa 2 B3ar Loop3 Or Xa 2 B3ar Tail, supplied by Promega, used in various techniques. Bioz Stars score: 90/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
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Average 90 stars, based on 1 article reviews
biotinylated fusion proteins for the wild-type or mutated xa-2-b3ar loop3 or xa-2-b3ar tail - by Bioz Stars, 2026-10
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pvhl antiserum  (Becton Dickinson)
90
Becton Dickinson pvhl antiserum
Pvhl Antiserum, supplied by Becton Dickinson, used in various techniques. Bioz Stars score: 90/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
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Average 90 stars, based on 1 article reviews
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protein precipitate solution  (Promega)
90
Promega protein precipitate solution
Protein Precipitate Solution, supplied by Promega, used in various techniques. Bioz Stars score: 90/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
https://www.bioz.com/product/mpc+protein+precipitation+solution/protein+precipitate+solution/pmc06491663-334-35-38
Average 90 stars, based on 1 article reviews
protein precipitate solution - by Bioz Stars, 2026-10
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200 μl protein precipitation solution  (Promega)
90
Promega 200 μl protein precipitation solution
200 μl Protein Precipitation Solution, supplied by Promega, used in various techniques. Bioz Stars score: 90/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
https://www.bioz.com/product/mpc+protein+precipitation+solution/wizard+hmw+dna+extraction+kit/bio_rxiv__2023__04__20__537689-110-9-12
Average 90 stars, based on 1 article reviews
200 μl protein precipitation solution - by Bioz Stars, 2026-10
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protein precipitation solution  (Promega)
90
Promega protein precipitation solution
Co-purification of chromosomal DNA fragments with native Smc–ScpAB. Cells of strains BSG1104-5 and BSG1107-8 were treated with the cross-linker BMOE prior to cell lysis. Strains carrying (‘+’) or lacking (‘−’) cysteine mutations (‘6xCys’) in the Smc-AviTag construct were expressed in presence (‘+’) or absence (‘−’) of the biotin ligase (‘BirA’). Beads were washed in the presence of either a 150 mM ammonium acetate buffer (‘low salt’) or a 2 M sodium chloride buffer (‘high salt’). The co-purification of DNA fragments with Smc-biotin on streptavidin beads was measured by quantitative PCR using primer pairs specific for genomic positions indicated on a representation of the circular B. subtilis genome. Mean values and standard deviations were calculated from two independent biological replicates. The following figure supplement is available: : Chromatin <t>immuno-precipitation</t> of Smc. DOI: http://dx.doi.org/10.7554/eLife.06659.012
Protein Precipitation Solution, supplied by Promega, used in various techniques. Bioz Stars score: 90/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
https://www.bioz.com/product/mpc+protein+precipitation+solution/protein+precipitation+solution/pmc04442127-390-3-11
Average 90 stars, based on 1 article reviews
protein precipitation solution - by Bioz Stars, 2026-10
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isolute c18 columns  (BIOTAGE)
90
BIOTAGE isolute c18 columns
Co-purification of chromosomal DNA fragments with native Smc–ScpAB. Cells of strains BSG1104-5 and BSG1107-8 were treated with the cross-linker BMOE prior to cell lysis. Strains carrying (‘+’) or lacking (‘−’) cysteine mutations (‘6xCys’) in the Smc-AviTag construct were expressed in presence (‘+’) or absence (‘−’) of the biotin ligase (‘BirA’). Beads were washed in the presence of either a 150 mM ammonium acetate buffer (‘low salt’) or a 2 M sodium chloride buffer (‘high salt’). The co-purification of DNA fragments with Smc-biotin on streptavidin beads was measured by quantitative PCR using primer pairs specific for genomic positions indicated on a representation of the circular B. subtilis genome. Mean values and standard deviations were calculated from two independent biological replicates. The following figure supplement is available: : Chromatin <t>immuno-precipitation</t> of Smc. DOI: http://dx.doi.org/10.7554/eLife.06659.012
Isolute C18 Columns, supplied by BIOTAGE, used in various techniques. Bioz Stars score: 90/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
https://www.bioz.com/product/mpc+protein+precipitation+solution/isolute+c18+columns/10__1161_slash_circulationaha__119__041868-22-13-15
Average 90 stars, based on 1 article reviews
isolute c18 columns - by Bioz Stars, 2026-10
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protein precipitation solution promega a795a  (Promega)
90
Promega protein precipitation solution promega a795a
Co-purification of chromosomal DNA fragments with native Smc–ScpAB. Cells of strains BSG1104-5 and BSG1107-8 were treated with the cross-linker BMOE prior to cell lysis. Strains carrying (‘+’) or lacking (‘−’) cysteine mutations (‘6xCys’) in the Smc-AviTag construct were expressed in presence (‘+’) or absence (‘−’) of the biotin ligase (‘BirA’). Beads were washed in the presence of either a 150 mM ammonium acetate buffer (‘low salt’) or a 2 M sodium chloride buffer (‘high salt’). The co-purification of DNA fragments with Smc-biotin on streptavidin beads was measured by quantitative PCR using primer pairs specific for genomic positions indicated on a representation of the circular B. subtilis genome. Mean values and standard deviations were calculated from two independent biological replicates. The following figure supplement is available: : Chromatin <t>immuno-precipitation</t> of Smc. DOI: http://dx.doi.org/10.7554/eLife.06659.012
Protein Precipitation Solution Promega A795a, supplied by Promega, used in various techniques. Bioz Stars score: 90/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
https://www.bioz.com/product/mpc+protein+precipitation+solution/protein+precipitation+solution+promega+a795a/pmc06476885-329-6-11
Average 90 stars, based on 1 article reviews
protein precipitation solution promega a795a - by Bioz Stars, 2026-10
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precision plus protein kaleidoscope standards  (OneLab Solutions)
99
OneLab Solutions precision plus protein kaleidoscope standards
Co-purification of chromosomal DNA fragments with native Smc–ScpAB. Cells of strains BSG1104-5 and BSG1107-8 were treated with the cross-linker BMOE prior to cell lysis. Strains carrying (‘+’) or lacking (‘−’) cysteine mutations (‘6xCys’) in the Smc-AviTag construct were expressed in presence (‘+’) or absence (‘−’) of the biotin ligase (‘BirA’). Beads were washed in the presence of either a 150 mM ammonium acetate buffer (‘low salt’) or a 2 M sodium chloride buffer (‘high salt’). The co-purification of DNA fragments with Smc-biotin on streptavidin beads was measured by quantitative PCR using primer pairs specific for genomic positions indicated on a representation of the circular B. subtilis genome. Mean values and standard deviations were calculated from two independent biological replicates. The following figure supplement is available: : Chromatin <t>immuno-precipitation</t> of Smc. DOI: http://dx.doi.org/10.7554/eLife.06659.012
Precision Plus Protein Kaleidoscope Standards, supplied by OneLab Solutions, used in various techniques. Bioz Stars score: 99/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
https://www.bioz.com/product/mpc+protein+precipitation+solution/Precision+Plus+Protein+Kaleidoscope+Standards/custom%401610375%4010%2E64898%2F2026%2E04%2E13%2E718024
Average 99 stars, based on 1 article reviews
precision plus protein kaleidoscope standards - by Bioz Stars, 2026-10
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tris hcl  (Bio-Rad)
98
Bio-Rad tris hcl
Co-purification of chromosomal DNA fragments with native Smc–ScpAB. Cells of strains BSG1104-5 and BSG1107-8 were treated with the cross-linker BMOE prior to cell lysis. Strains carrying (‘+’) or lacking (‘−’) cysteine mutations (‘6xCys’) in the Smc-AviTag construct were expressed in presence (‘+’) or absence (‘−’) of the biotin ligase (‘BirA’). Beads were washed in the presence of either a 150 mM ammonium acetate buffer (‘low salt’) or a 2 M sodium chloride buffer (‘high salt’). The co-purification of DNA fragments with Smc-biotin on streptavidin beads was measured by quantitative PCR using primer pairs specific for genomic positions indicated on a representation of the circular B. subtilis genome. Mean values and standard deviations were calculated from two independent biological replicates. The following figure supplement is available: : Chromatin <t>immuno-precipitation</t> of Smc. DOI: http://dx.doi.org/10.7554/eLife.06659.012
Tris Hcl, supplied by Bio-Rad, used in various techniques. Bioz Stars score: 98/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
https://www.bioz.com/product/mpc+protein+precipitation+solution/Tris/pmc03446618-42-16-28
Average 98 stars, based on 1 article reviews
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protein a-sepharose cl-4b  (Pharmacia LKB Biotechnology Inc)
90
Pharmacia LKB Biotechnology Inc protein a-sepharose cl-4b
Co-purification of chromosomal DNA fragments with native Smc–ScpAB. Cells of strains BSG1104-5 and BSG1107-8 were treated with the cross-linker BMOE prior to cell lysis. Strains carrying (‘+’) or lacking (‘−’) cysteine mutations (‘6xCys’) in the Smc-AviTag construct were expressed in presence (‘+’) or absence (‘−’) of the biotin ligase (‘BirA’). Beads were washed in the presence of either a 150 mM ammonium acetate buffer (‘low salt’) or a 2 M sodium chloride buffer (‘high salt’). The co-purification of DNA fragments with Smc-biotin on streptavidin beads was measured by quantitative PCR using primer pairs specific for genomic positions indicated on a representation of the circular B. subtilis genome. Mean values and standard deviations were calculated from two independent biological replicates. The following figure supplement is available: : Chromatin <t>immuno-precipitation</t> of Smc. DOI: http://dx.doi.org/10.7554/eLife.06659.012
Protein A Sepharose Cl 4b, supplied by Pharmacia LKB Biotechnology Inc, used in various techniques. Bioz Stars score: 90/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
https://www.bioz.com/product/mpc+protein+precipitation+solution/cnbr+activated+sepharose+4b/10__1042_slash_bj2690709-50-44-47
Average 90 stars, based on 1 article reviews
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precision plus protein dual xtra strandard  (Bio-Rad)
99
Bio-Rad precision plus protein dual xtra strandard
Co-purification of chromosomal DNA fragments with native Smc–ScpAB. Cells of strains BSG1104-5 and BSG1107-8 were treated with the cross-linker BMOE prior to cell lysis. Strains carrying (‘+’) or lacking (‘−’) cysteine mutations (‘6xCys’) in the Smc-AviTag construct were expressed in presence (‘+’) or absence (‘−’) of the biotin ligase (‘BirA’). Beads were washed in the presence of either a 150 mM ammonium acetate buffer (‘low salt’) or a 2 M sodium chloride buffer (‘high salt’). The co-purification of DNA fragments with Smc-biotin on streptavidin beads was measured by quantitative PCR using primer pairs specific for genomic positions indicated on a representation of the circular B. subtilis genome. Mean values and standard deviations were calculated from two independent biological replicates. The following figure supplement is available: : Chromatin <t>immuno-precipitation</t> of Smc. DOI: http://dx.doi.org/10.7554/eLife.06659.012
Precision Plus Protein Dual Xtra Strandard, supplied by Bio-Rad, used in various techniques. Bioz Stars score: 99/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
https://www.bioz.com/product/mpc+protein+precipitation+solution/Precision+Plus+Protein+Dual+Xtra+Prestained+Protein+Standards/pm23509980__cn300159e_si_001-15-3-13
Average 99 stars, based on 1 article reviews
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perfect focus kit  (G Biosciences)
90
G Biosciences perfect focus kit
Co-purification of chromosomal DNA fragments with native Smc–ScpAB. Cells of strains BSG1104-5 and BSG1107-8 were treated with the cross-linker BMOE prior to cell lysis. Strains carrying (‘+’) or lacking (‘−’) cysteine mutations (‘6xCys’) in the Smc-AviTag construct were expressed in presence (‘+’) or absence (‘−’) of the biotin ligase (‘BirA’). Beads were washed in the presence of either a 150 mM ammonium acetate buffer (‘low salt’) or a 2 M sodium chloride buffer (‘high salt’). The co-purification of DNA fragments with Smc-biotin on streptavidin beads was measured by quantitative PCR using primer pairs specific for genomic positions indicated on a representation of the circular B. subtilis genome. Mean values and standard deviations were calculated from two independent biological replicates. The following figure supplement is available: : Chromatin <t>immuno-precipitation</t> of Smc. DOI: http://dx.doi.org/10.7554/eLife.06659.012
Perfect Focus Kit, supplied by G Biosciences, used in various techniques. Bioz Stars score: 90/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
https://www.bioz.com/product/mpc+protein+precipitation+solution/perfect+focus+kit/pmc06097156-617-5-8
Average 90 stars, based on 1 article reviews
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Image Search Results


Co-purification of chromosomal DNA fragments with native Smc–ScpAB. Cells of strains BSG1104-5 and BSG1107-8 were treated with the cross-linker BMOE prior to cell lysis. Strains carrying (‘+’) or lacking (‘−’) cysteine mutations (‘6xCys’) in the Smc-AviTag construct were expressed in presence (‘+’) or absence (‘−’) of the biotin ligase (‘BirA’). Beads were washed in the presence of either a 150 mM ammonium acetate buffer (‘low salt’) or a 2 M sodium chloride buffer (‘high salt’). The co-purification of DNA fragments with Smc-biotin on streptavidin beads was measured by quantitative PCR using primer pairs specific for genomic positions indicated on a representation of the circular B. subtilis genome. Mean values and standard deviations were calculated from two independent biological replicates. The following figure supplement is available: : Chromatin immuno-precipitation of Smc. DOI: http://dx.doi.org/10.7554/eLife.06659.012

Journal: eLife

Article Title: SMC condensin entraps chromosomal DNA by an ATP hydrolysis dependent loading mechanism in Bacillus subtilis

doi: 10.7554/eLife.06659

Figure Lengend Snippet: Co-purification of chromosomal DNA fragments with native Smc–ScpAB. Cells of strains BSG1104-5 and BSG1107-8 were treated with the cross-linker BMOE prior to cell lysis. Strains carrying (‘+’) or lacking (‘−’) cysteine mutations (‘6xCys’) in the Smc-AviTag construct were expressed in presence (‘+’) or absence (‘−’) of the biotin ligase (‘BirA’). Beads were washed in the presence of either a 150 mM ammonium acetate buffer (‘low salt’) or a 2 M sodium chloride buffer (‘high salt’). The co-purification of DNA fragments with Smc-biotin on streptavidin beads was measured by quantitative PCR using primer pairs specific for genomic positions indicated on a representation of the circular B. subtilis genome. Mean values and standard deviations were calculated from two independent biological replicates. The following figure supplement is available: : Chromatin immuno-precipitation of Smc. DOI: http://dx.doi.org/10.7554/eLife.06659.012

Article Snippet: 200 μl of ‘Protein Precipitation Solution’ (Wizard Genomic DNA Purification Kit, Promega) was added to each sample followed by a 10 s vortexing step and 5 min incubation on ice.

Techniques: Copurification, Lysis, Construct, Real-time Polymerase Chain Reaction, Chromatin Immunoprecipitation